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10x Genomics Single-cell Immunolibrary Sequencing
Brief

Annoroad Genome's 10x Genomics automated cell sorting platform based on microfluidic technology enables reverse transcription and amplification of single cell 5' transcripts, and simultaneous preparation of single cell 5' transcriptome library and immune library V(D)J library. The 10x Genomics platform enables automatic sorting of up to 80,000 cells in 7 minutes. Each GEM contains a single cell and reverse transcription reaction system, which can simultaneously obtain single cell 5 'gene expression data and TCR/BCR V(D)J rearrangement information, in-depth study of the complexity of cellular immune response, so that it has a wide application prospect in immunology and immuno-oncology.

Fields
  • Basic immunology
  • Immuno-oncology and immunotherapy
  • Autoimmune and inflammatory diseases
  • Infectious disease and vaccine research
  • Organ transplantation and immune reconstitution
Process

9、Single-cell sequencing – 10x Genomics single-cell immunolibrary sequencing.png

Note: Single-cell 5' transcriptome analysis process is shown on the left, and immuno-library V(D)J analysis process is shown on the right

Requirements

Sampling Requirements
Sample TypeCell suspension of fresh intact cells in vivo; Cell cryoprotectant; Samples not suitable for fixation, staining, etc.; Species are limited to human, C57BL/6 mice and BALB/ C mice, while V (D) J cell types are limited to T cells and B cells
Total Amount of SampleTotal amount >6*105 PCS; 2 copies of the same sample are prepared in parallel ( one for inspection and one for experiment ).
Sample Concentration700-1200 cells/μL
Sample QualityCells should be free of clumps, with less fragments, survival rate >90%, cell diameter< 30 μm, and free of reverse transcription inhibitors and non-cellular nucleic acid molecules
Cell Cryoprotectant SamplePlease consult local dealers for details
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