Iso-seq refers to the use of third-generation sequencing technology to sequence complete RNA molecules in organisms (including the full-length sequence from the 5' end to the 3' poly A tail). The RNA molecule does not need to be interrupted, and the complete sequence can be directly obtained by sequencing. More accurate transcript information can be obtained, and more variable splicing sites, new genes and new isomers, and fusion genes can be identified through Iso-seq.
| Product Type | Sequencing Strategy | Recommended Data Amount |
| Full-length Transcriptome | Construct a 1-10kb iso-seq library | 30 Gb subreads; 50 Gb subreads |
| Sampling Requirements | |
| Total RNA | ≥2μg |
| RNA Concentration | ≥230 ng/μl |
| RNA Purity | OD260/280: 2.0-2.2 |
| OD260/230: 1.8-2.1 | |
| Other Requirements | RIN≥7.2; 28s:18s≥1.0 |
| Do not freeze and thaw repeatedly | |
| Do not get exposed to high temperature (>65°C) | |
| Do not get exposed to high and low pH (<6 or ≥ 9) |
| Sample Type | Recommended Sample Size(No Biological Replicates Required) | Recommended Site |
| Plant Tissue | ≥3-4g | Tender tissue |
| Animal Tissue | ≥1-2g | Viscera, gills, etc. |
| Cell | ≥1x107 cells/library | - |