The research object of eukaryotic mRNA sequencing analysis is the sum of all mRNAs that can be transcribed by a specific cell in a certain functional state. Transcriptome research is the basis and starting point for the study of gene function and structure. Through the new generation of high-throughput sequencing, almost all the transcriptome sequence information of a specific tissue or organ of a species in a certain state can be obtained.
| Product Type | Sequencing Strategy | Indicator | Cycle |
| Eukaryotic Transcriptome Sequencing | NovaSeq 6000 PE150 DNBSEQ-T7 PE150 | With reference: 6/8/10 G clean data | 16 days |
| Without reference: 6/8/10 G clean data | 26 days |
| Product Type | Sampling Requirements | |
| Transcriptome Sequencing | Requirement | Plant samples: ≥ 2 μg Animal sample: ≥ 1μg Insect sample: ≥ 1μg |
| Concentration | Plant samples: ≥60 ng/μl Animal sample: ≥30 ng/μl Insect sample: ≥30 ng/μl | |
| Purity | OD260/OD280=1.8-2.2, OD260/230 for special anomalies (eg. negative values, exceeding 2.5) is not included in the judgment criteria | |
| RNA Integrity | Plant samples: RIN≥6.5 Animal sample: RIN≥7 Insect sample: RIN≥7 | |