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LncRNA Sequencing
Brief

Long non-coding RNA (Long non-coding RNA) is a class of RNA with a length of more than 200 bp, which does not encode proteins, and widely exists in living organisms. Library construction is fulfilled by utilizing rRNA removal chain specificity, and the lncRNA sequence information of single-base resolution is obtained at one time by high-throughput sequencing technology. Known lncRNAs are identified and new lncRNAs and their targets are predicted based on the bioinformatics analysis platform.

Fields
  • Environmental stress
  • Transcriptional regulation
  • Drug development
  • Molecular markers
  • Growth and development
Process
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Parameters
Product TypeStrategyRecommended Data SizeCycle
LncRNA Sequencing

Illumina PE150

DNBSEQ-T7 PE150

12G clean data / 24G clean data36 days
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Requirements
Product TypeSampling Requirements
LncRNA SequencingRequirement

Samples for human, rat, and mouse: ≥ 1 μg

Samples for plant leaves and stems: ≥ 2μg

Samples for other plants and microorganisms: ≥ 2μg

Concentration

Samples for human, rat, and mouse: ≥100 ng/μl

Samples for plant leaves and stems 100 ng/μl

Samples for other plants and microorganisms: 100 ng/μl

PurityOD260/OD280=1.8-2.2
RNA Integrity

Plant samples: RIN≥6.5

Animal sample: RIN≥7

Insect sample: RIN≥7

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