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Whole Transcriptome Sequencing
Brief

Whole transcriptome refers to the sum of all transcript information transcribed by a specific tissue or cell in a specific state, including mRNA, small RNA, lncRNA, circRNA and other coding and non-coding RNA. Four kinds of RNA-level analysis can be realized by constructing lncRNA library and sequencing small RNA library, and ceRNA joint analysis is performed combining with 4 kinds of RNA information, and to explore the underlying regulatory network mechanisms using the whole transcriptome sequencing technology has become a powerful tool to explain biological phenomena!

Fields
  • Environmental stress
  • Transcriptional regulation
  • Drug development
  • Molecular markers
  • Growth and development
Requirements
Product TypeSampling Requirements
Whole Transcriptome SequencingRequirement

Samples for human, rat, and mouse: ≥ 1 μg

Samples for plant leaves and stems: ≥ 2μg

Samples for other plants and microorganisms: ≥ 2μg

Concentration

Samples for human, rat, and mouse: ≥100 ng/μl

Samples for plant leaves and stems 100 ng/μl

Samples for other plants and microorganisms: 100 ng/μl

PurityOD260/OD280=1.8~2.2
RNA Integrity

Plant samples: RIN≥6.5

Animal sample: RIN≥7

Insect sample: RIN≥7

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