Whole transcriptome refers to the sum of all transcript information transcribed by a specific tissue or cell in a specific state, including mRNA, small RNA, lncRNA, circRNA and other coding and non-coding RNA. Four kinds of RNA-level analysis can be realized by constructing lncRNA library and sequencing small RNA library, and ceRNA joint analysis is performed combining with 4 kinds of RNA information, and to explore the underlying regulatory network mechanisms using the whole transcriptome sequencing technology has become a powerful tool to explain biological phenomena!
Brief
Fields
- Environmental stress
- Transcriptional regulation
- Drug development
- Molecular markers
- Growth and development
Requirements
Product Type | Sampling Requirements | |
Whole Transcriptome Sequencing | Requirement | Samples for human, rat, and mouse: ≥ 1 μg Samples for plant leaves and stems: ≥ 2μg Samples for other plants and microorganisms: ≥ 2μg |
Concentration | Samples for human, rat, and mouse: ≥100 ng/μl Samples for plant leaves and stems: ≥100 ng/μl Samples for other plants and microorganisms: ≥100 ng/μl | |
Purity | OD260/OD280=1.8~2.2 | |
RNA Integrity | Plant samples: RIN≥6.5 Animal sample: RIN≥7 Insect sample: RIN≥7 |
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